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yeast cell [-plant] 酵母,酵母菌。

yeast powder

We used fission yeast schizosaccharomyces pombe ( s . pombe ) , an unicellular eukaryotic organism , as research material . electroporation was adopted to load ca2 + fluorescent indicator into yeast cell and under the laser scanning confocal microscopy ( lscm ) , we observed cytosolic ca2 + distribution and relative content as well as fluorescence intensity of gfp - cam in different phases of cell cycle of yeast cell . flow cytometry provided a way of determining the relative dna content of populations of fission yeast 本文以單細胞的真核模式生物裂殖酵母( schizosaccharomycespombe )為研究材料,通過激光掃描共聚焦顯微鏡觀察酵母細胞胞質內游離ca ~ ( 2 + )的分布及相對濃度,以及不同周期時相細胞中gfp - cam的熒光強度變化,并采用細胞流式法對酵母細胞的相對dna含量進行測定以確定細胞所處周期時相。

In our work , different hbv pres gene fragments were amplified by pcr , cloned into pgbkt ? vector in yeast two - hybrid system 3 , and then transformed ah 109 yeast cells . the results showed that these pres expression products were nontoxic to yeast cells , but all self - activated the yeast two - hybrid system 本研究將hbv前s區不同片斷進行擴增,克隆入酵母雙雜交系統3中的pgbkt7載體,轉化酵母細胞ah109 ,經檢測,證實了hbv前s區不同片斷對酵母細胞無毒性作用,但是都存在自激活作用。

Multistage fluidized - bed bioreactor with external circulation has been in use in the production of fuel alcohol by immobilized yeast cells technique . as a new - style reactor . it has the advantage of high conversion , complete reaction and little damage to immobilized yeast cells . flow properties is the foundation of reactor ' s physical transmission process . and it is the key factor of deciding whether the auxiliary material conversion is high or low . at present , the study on flow properties of multistage fluidized - bed bioreactor with external circulation at a pilot scale is rarely reported 多級外循環流化床生物反應器作為一種新型生物反應器,由于具有轉化率高、反應徹底、對顆粒損傷少等優點在固定化細胞制取燃料酒精技術上得到應用。但是,國內關于中試規模多級外循環流化床反應器流動特性的研究還很少,而流動特性是反應器物理傳遞過程的基礎,是研究物料在反應器中轉化率高低的重要因素,因此,弄清楚外循環流化床反應器的流動特性是非常重要的。

In order to study the mechanism of the effect of low concentration tfp on the proliferation of s . pombe , we watch yeast cells loaded with fluo - 3 under laser scanning confocal microscope ( lscm ) . the fluorescence intensity reflected the cytosolic free calcium concentration . the result showed that , the cytosolic free ca2 + concentration in s . pombe cultured in ca2 + - free medium was 2 ~ 3 times lower than that in s . pombe cultured in medium containing 10umol / l ca24 , while ca2 + concentration in s . pombe treated with 50umol / l tfp was 4 - 5 times higher 本文發現增加胞外鈣濃度以及低濃度( 20 100 mol l )三氟拉嗪( tfp )不但能促進野生型s . pombe細胞的增殖,而且對mfp7菌株也有同樣的效應,這說明胞外ca ~ ( 2 + )和低濃度tfp對不同遺傳型裂殖酵母細胞的增殖均有促進作用。

The right arm vector is constructed with arabidopsis telomere sequence , kanmx4 gene of g418 selection marker and truncated trpi gene . the left arm vector carries arabidopsis telomere sequence , ade2 gene of selection marker , ars sequence , gfp reporter gene , and truncated ura3 gene . retrofitting yac clones carrying arabidopsis centromere segment by homologous recombination with the constructed right and left arm vectors was performed and confirmed in yeast , thus the rudiment of plant artificial chromosome has been formed in yeast cells 與右臂同源重組的載體包含了端粒,用于在酵母中篩選的g418的抗性基因kanmx4和用于與yacdna同源重組的trp1基因片段;華中農業大學碩l學位論文2的3年5月與左臂同源重組的載體包含了端粒,用于在酵母中篩選的a瓏2基因,用于在植物中篩選的份畢萬基因,用于同源重組的乙厲洲3基因片段和人rs片段。

Different strains of yeast were separated from the vary origin distiller ' s yeast , the cellular forms , cellular size , fermenting power , yeast cells suspended number , total sugar , alcohol degrees , cohesion , lethal temperature and alcohol tolerance among the strains were compared 摘要隨機分離黃酒生產用酒餅中的酵母菌菌株,比較不同來源的酵母菌細胞形態、細胞大小、發酵力、酵母懸浮細胞數、總降糖、酒精度、凝聚力、致死溫度和耐酒精度,研究黃酒生產中酵母菌的發酵性能。

High concentration of tfp can enter yeast cells and combine to cam to make it inactive , which is the reason that tfp restrain the growth of yeast cells . 5mmol / l egta could completely arrest the cell proliferation of mfp7 after 28h , when the fluorescence intensity in cells was obviously increased with flow cytometry and lscm 被較高濃度tfp處理過的mfp7胞內熒光強度則相對較弱,推測是因為tfp進入胞內與cam結合從而使其失活,這也是高濃度tfp抑制酵母細胞增殖的原因。

The article mainly studied and analyzed the chemical structure and conponents of yeast , pointed out that the way that yeast autolysis combined together with both breaking - wall in temperature difference and high - pressure homogenization can be used to promot the inside things from waste yeast cell and enhance the extract rate , thus searched for the technology in higher extraction rate 摘要主要研究分析了酵母化學結構與組成,提出了酵母自溶、溫差破壁、高壓均漿三者相結合的方法來促進酵母內容物溶出提高抽提率,探索抽提率比較高的工藝流程。

This paper reviews the recent advances in yeast expression systems used in recombinant protein drugs produced by gene engineering , such as the selection of yeast vector system and yeast expression strain , construction of multi - copy strain , the high yeast cell density culture , the recent situation of yeast system applied in expression gene , defect of yeast expression system and countermeasure , etc 摘要本文主要從酵母載體系統的選擇、酵母表達宿主的選擇、多拷貝菌株的構建、高密度發酵培養酵母細胞、應用酵母系統表達外源基因的研究現狀、酵母表達系統的缺陷及對策等幾個方面綜述了近年來酵母表達體系在基因工程重組蛋白藥物開發方面的研究進展。

Protein and nucleic acid in yeast cells were degraded , then the product was refined into nutritional seasoning , which was in step with current tide of nutrition and nature 摘要酵母抽提物亦稱酵母味素,是將酵母細胞內蛋白質、核酸等進行生物降解,經加工而成的營養型天然調味料,符合目前“天然、營養、回歸自然”之潮流。

2 ) the growth curves for different cells show that overexpression of gpil7p has no lethal effect on the yeast cells and only that of the dominant negative mutants can cause cells not grow 2 )生長曲線的測定表明,過表達gpi17p對正常酵母細胞不致死,而只有顯性負性突變體的過表達才會導致酵母細胞不能生長。

The result shows that mms can induce dna - damage of yeast cells and the situation of dna - damage aggravated with increase of mms concentration 結果顯示mms能夠引起酵母細胞dna的損傷,并且隨著mms濃度的增加dna損傷程度加重, 0 . 5 %瓊脂糖凝膠電泳及eb染色顯示1 ~ 。

This model organism can be as simple as a yeast cell or as complex as a nematode worm , fruitfly , or even a mouse 這一生物模型可以是簡單的酵母細胞或復雜的線蟲甚至老鼠。

In the yeast cell , a bulbous projection , the bud , appears at one portion of the cell wall 在酵母細胞中球莖狀的突出物- -芽體出現在一部分細胞壁的上面。

Study on the estrogenic activities of ddt pesticides based on the gene recombinant yeast cell 結論嘉陵江水中有機污染物可誘導大鼠肝細胞芳烴受體

Glutathione biosynthesis by the immobilized yeast cell with - carrgeenin - konjac flour 卡拉膠與魔芋粉混合載體固定酵母細胞生物合成谷胱甘肽

Yeast cell activation method of hemaimmune reaction road map experimental system 血液免疫反應路線圖實驗系統酵母多糖激活法